Assembly on a Scaffold
For the design of a "superantibody" the development of scaffold structures where peptides or glycans can be bound is necessary. These scaffold structures must be balanced between flexibility and stability. Flexibility is essential to allow a manifold combination of multiple recognition domains. But the anchorage of recognition and signalling elements is only possible on stable matrices.
The basis of the used polymer scaffold are functional nonbiodegradable hydrogels. The flexible design of the scaffold systems and connecting elements leads to a highly variable platform that can be adapted to the recognition of different pathogens. Independent from the signal generation by downstream cascades the development of hydrogels that change their physical properties after binding of the analytes could lead to polymer scaffolds with integrated signal generation.